Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Absorption Spectroscopy: Atomization Methods01:25

Atomic Absorption Spectroscopy: Atomization Methods

Atomic Absorption Spectroscopy (AAS) atomizes samples through flame atomization or electrothermal atomization. Flame atomization typically involves a nebulizer and spray chamber assembly to combine the sample with a fuel–oxidant mixture, creating a fine aerosol mist that enters a burner. Typically, the fuel and oxidant are combined in an approximately stoichiometric ratio. However, for atoms that are easily oxidized, a fuel-rich mixture may be more advantageous. Only about 5% of the aerosol...
Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Atomic Absorption Spectroscopy: Lab01:21

Atomic Absorption Spectroscopy: Lab

For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
 Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then passed on to...
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
Atomic Absorption Spectroscopy: Interference01:25

Atomic Absorption Spectroscopy: Interference

Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pineal indoles stimulate the gene expression of immunomodulating cytokines.

Journal of neural transmission (Vienna, Austria : 1996)·2001
Same author

Transfer of full-length Dmd to the diaphragm muscle of Dmd(mdx/mdx) mice through systemic administration of plasmid DNA.

Molecular therapy : the journal of the American Society of Gene Therapy·2001
Same author

Synthesis of a new class of 5'-functionalized adenosines using a rh(ii)-catalyzed 1,3-dipolar cycloaddition.

Organic letters·2001
Same author

The role of factor XI in a dilute thromboplastin assay of extrinsic coagulation pathway.

Thrombosis and haemostasis·2001
Same author

Study on sludge expansion during treatment of salad oil manufacturing wastewater by yeast.

Environmental technology·2001
Same author

Transfection of the c-erbB2/neu gene upregulates the expression of sialyl Lewis X, alpha1,3-fucosyltransferase VII, and metastatic potential in a human hepatocarcinoma cell line.

European journal of biochemistry·2001
Same journal

Efficient methods for wave propagation in electron microscopy.

Ultramicroscopy·2026
Same journal

Unsupervised deep image prior for sparse-view and limited-angle electron tomography.

Ultramicroscopy·2026
Same journal

Determination of the structure of the tertiary phase in the alloy Al<sub>10</sub>Mo<sub>10</sub>Nb<sub>10</sub>Ta<sub>10</sub>Ti<sub>30</sub>Zr<sub>30</sub> using convergent beam electron diffraction.

Ultramicroscopy·2026
Same journal

Predictive drift compensation of multi-frame STEM via live scan modification.

Ultramicroscopy·2026
Same journal

Deep PACBED: Multitask analysis of PACBED images using deep neural networks.

Ultramicroscopy·2026
Same journal

Guided progressive reconstructive imaging: A new quantization-based framework for low-dose, high-throughput and real-time analytical ptychography.

Ultramicroscopy·2026
See all related articles

Related Experiment Video

Updated: Jun 5, 2026

Processing of Bulk Nanocrystalline Metals at the US Army Research Laboratory
08:58

Processing of Bulk Nanocrystalline Metals at the US Army Research Laboratory

Published on: March 7, 2018

Quantitative atom probe analysis of carbides.

M Thuvander1, J Weidow, J Angseryd

  • 1Chalmers University of Technology, Göteborg, Sweden. mattias.thuvander@chalmers.se

Ultramicroscopy
|January 18, 2011
PubMed
Summary
This summary is machine-generated.

Atom probe tomography often underestimates carbon concentration in carbides due to detector limitations. Analyzing the carbon-13 isotope improves accuracy by accounting for frequent multiple ion events.

More Related Videos

Atom Probe Tomography Studies on the Cu(In,Ga)Se2 Grain Boundaries
09:51

Atom Probe Tomography Studies on the Cu(In,Ga)Se2 Grain Boundaries

Published on: April 22, 2013

Atom Probe Tomography Analysis of Exsolved Mineral Phases
08:14

Atom Probe Tomography Analysis of Exsolved Mineral Phases

Published on: October 25, 2019

Related Experiment Videos

Last Updated: Jun 5, 2026

Processing of Bulk Nanocrystalline Metals at the US Army Research Laboratory
08:58

Processing of Bulk Nanocrystalline Metals at the US Army Research Laboratory

Published on: March 7, 2018

Atom Probe Tomography Studies on the Cu(In,Ga)Se2 Grain Boundaries
09:51

Atom Probe Tomography Studies on the Cu(In,Ga)Se2 Grain Boundaries

Published on: April 22, 2013

Atom Probe Tomography Analysis of Exsolved Mineral Phases
08:14

Atom Probe Tomography Analysis of Exsolved Mineral Phases

Published on: October 25, 2019

Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Surface Science

Background:

  • Atom probe tomography (APT) is a powerful technique for analyzing materials at the atomic scale.
  • Carbide phases present unique challenges in APT due to molecular ion formation and multiple detection events.
  • Standard APT methods often lead to an underestimation of carbon concentration in these materials.

Purpose of the Study:

  • To investigate the underestimation of carbon concentration during APT analysis of various carbide phases.
  • To identify the primary causes of inaccurate carbon quantification in materials like SiC, WC, Ti(C,N), and Ti(2)AlC.
  • To develop improved methods for accurate carbon measurement in carbide materials using APT.

Main Methods:

  • Laser-pulsed atom probe tomography was employed to analyze SiC, WC, Ti(C,N), Ti(2)AlC, and M(23)C(6) precipitates.
  • Standard data evaluation methods were initially used to assess carbon concentration.
  • A refined method involving the selective analysis of the (13)C isotope and calculation of (12)C concentration from natural abundance was implemented.

Main Results:

  • Standard APT evaluation resulted in a 6-24% lower carbon concentration than expected based on material stoichiometry.
  • The underestimation is primarily attributed to detector dead time, exacerbated by frequent multiple ion events of carbon.
  • Mass-to-charge overlaps, such as C(2)(+), C(4)(2+), and Ti(2+) at 24 Da, add complexity to Ti(C,N) and Ti(2)AlC analysis.
  • Utilizing the (13)C isotope significantly improved the accuracy of carbon concentration measurements.

Conclusions:

  • Detector dead time is a critical factor causing carbon underestimation in APT of carbides.
  • The frequency of multiple ion events for carbon significantly impacts measurement accuracy.
  • Analyzing specific isotopes, like (13)C, and accounting for natural abundance provides a more reliable method for carbon quantification in complex carbide materials.
  • Further methodological refinements are necessary to overcome mass overlaps in specific carbide systems.