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Age estimation using cytochrome c oxidase activity analysis.

Noboru Ishikawa1, Junpei Hara, Tomokazu Takasaka

  • 1Department of Forensic Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kawaramachi, Kamigyo, Kyoto, Japan.

Forensic Science International
|January 18, 2011
PubMed
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Forensic scientists can estimate age using cytochrome c oxidase (CCO) activity. This mitochondrial enzyme

Area of Science:

  • Forensic Medicine
  • Biochemistry
  • Aging Research

Background:

  • Increasing unidentified cadavers necessitate advanced identification methods.
  • Mitochondrial activity declines with age, linked to oxidative stress.
  • Cytochrome c oxidase (CCO) is a key mitochondrial enzyme.

Purpose of the Study:

  • To investigate CCO activity as a potential biomarker for age estimation in forensic cases.
  • To analyze CCO protein and mRNA expression in aging rats.
  • To validate CCO activity for human age estimation.

Main Methods:

  • Analysis of cytochrome c oxidase (CCO) activity, protein, and mRNA in rats of various ages.
  • Measurement of human CCO activity in 28 forensic cases.
  • Correlation of CCO activity with chronological age.

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Main Results:

  • CCO activity, protein, and mRNA levels in rats peaked at 3 weeks and declined thereafter.
  • Human CCO activity demonstrated a gradual decrease with increasing age.
  • A significant correlation was observed between CCO activity and age in human subjects.

Conclusions:

  • CCO activity serves as a reliable indicator for age estimation in forensic contexts.
  • This method offers a precise alternative to conventional anthropological techniques.
  • Mitochondrial function analysis provides valuable insights into the aging process.