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Cell adhesion to borate glasses by colloidal probe microscopy.

Sheldon M Wiederhorn1, Young-Hun Chae, Carl G Simon

  • 1Ceramics Division/Materials Science and Engineering Laboratory, National Institute of Standards and Technology, Gaithersburg, MD, USA. sheldon.wiederhorn@nist.gov

Acta Biomaterialia
|January 19, 2011
PubMed
Summary
This summary is machine-generated.

Osteoblast-like cell adhesion to silicate and borate glasses increases with contact time, suggesting a diffusion-limited process. Stronger adhesion correlates with calcium phosphate layer formation on glass surfaces.

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Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Surface Chemistry

Background:

  • Osteoblast adhesion is crucial for bone integration of biomaterials.
  • Understanding cell-glass interactions informs the design of bioactive glasses.

Purpose of the Study:

  • To quantify osteoblast-like cell adhesion forces to silicate and borate glasses.
  • To investigate the influence of glass composition and contact time on cell adhesion.
  • To elucidate the mechanisms underlying cell adhesion to bioactive glasses.

Main Methods:

  • Colloidal probe microscopy was used to measure adhesion forces between osteoblast-like cells and glass spheres.
  • Glass composition and cell-glass contact time (15-900 s) were systematically varied.
  • Atomic force microscopy (AFM) with functionalized cantilevers was employed.

Main Results:

  • Adhesive force increased with contact time, exhibiting a square root dependence in most cases.
  • A diffusion-limited process, potentially involving integrin diffusion, is suggested for early cell attachment.
  • Glasses forming a calcium phosphate reaction layer showed significantly stronger cell adhesion.

Conclusions:

  • Cell adhesion to bioactive glasses is a time-dependent, diffusion-controlled process.
  • The formation of a calcium phosphate layer is critical for strong osteoblast adhesion.
  • These findings support the role of surface reactions in the bioactivity of silicate and borate glasses.