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[Clone and construction of plant expression vector of AeSS gene in Aralia elata]
Ying Wu1, Hui Cheng, Hong-Da Zou
1College of Plant Science, Jilin University, Changchun 130062, China. wuying35969@126.com
Objective:
To clone Aralia elata squalene synthase gene (designated as AeSS) and construct plant expression vector for transgenic research.
Methods:
Isolated squalene synthase from Aralia elata with specific primers by RT-PCR and inserted AeSS gene into the plant expression vector pBI121.
Results:
The full-length cDNA of AeSS (Genebank accession Number: GU354313) was 1 261 bp and contained a 1 245 bp open reading frame (ORF) encoding a polypeptide of 414 amino acids. The plant expression vector pAeSS was constructed by inserted AeSS gene into the downstream of 35 S promoter of plant expression vector pBI121.
Conclusion:
AeSS gene was cloned and plant expression vector was constructed for future research.
