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[Study on the purification and scavenging free radical activity of water soluble polysaccharide from Ligularia
Chun-Lan Liu1, Yang Li, Ning Du
1College of Life and Environmental Science, MINZU University of China, Beijing 100081, China. liuchunlan888@yahoo.com.cn
Objective:
The study was to provided the reference for further development and utilization of Ligularia hodgsonii.
Methods:
The crude polysaccharide LW was extracted from Lugularia hodgsonii with hot water, and prectated by ethanol. The crude polysaccharide has been fractionted and purified, so LW21 was got. Its homogeneity was confirmed by chromatography on Sepharose CL-4B, paper chromatography and cellulous actate electrophoresis. The scavenging effets of the polysacehardes on * OH and O2-* were determined by fluorescence method.
Results:
Antioxidative experiment shows that LW and LW21 were effective in scavenging superoxide radical and hydroxyl radical. The scavenging capacity for superoxide radical and hydroxyl radical of LW21 had higher than that of LW.
Conclusion:
The polysaceharide from Ligularia hodgsonii had stronger antioxidant actioxdant activities, and their antioxidant activities had certain dose-relationship.
