Related Experiment Videos
[Relationship between protein kinase CβI(PKC-βI) expression, apoptosis and prognosis in patients with non-small cell
Xi Cheng1, Qinghua Zhou, Yun Wang
1Key Laboratory of Lung Cancer Molecular Biology of Sichuan Province and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R.China.
Background:
To investigate the relationship between expression of PKC-βI, apoptosis and prognosis of non-small cell lung cancer (NSCLC).
Methods:
The expression of PKC-βI and apoptosis index (AI) were detected in 119 human NSCLC tissues and paracancerous tissues by LSAB and TUNEL, with 32 benign pulmonary disease tissues as control.
Results:
(1)The expression of PKC-βI (82.27%) in NSCLC tissues was significantly higher than those (62.85% and 50.47%) in paracancerous tissues and benign pulmonary disease tissues (P < 0.05). The AI (5.27%) in NSCLC tissues was significantly lower than that ( 15.84%) in benign pulmonary disease tissues (P < 0.05). (2) No significant relationship was observed between the expression of PKC-βI and clinical physiopathological characteristics of NSCLC (P > 0.05). The AI was closely related to pTNM stage of the cancer, size of primary tumor and lymph node metastasis (P < 0.05), but not to the histological classification, cell differentiation, sex and age of the patients with NSCLC (P > 0.05). (3) A highly significant negative correlation was observed between PKC-βI expression and AI in NSCLC group (P < 0.01). (4) The 5-year survival rate (7.37%) in patients with high PKC-βI expression was much lower than that (37.06%) in patients with low PKC-βI expression (P < 0.01). The 5-year survi-val rate ( 39.24%) in patients with high AI was much higher than that (6.14%) in patients with low AI (P < 0.01).
Conclusions:
The abnormal activation of PKC-βI and suppression of apoptosis may play important roles in the oncogenesis and prognosis of lung cancer. Detection of PKC-βI expression and AI may help to predict the prognosis of patients with NSCLC and guide the postoperative multimodality therapy.
Related Concept Videos
The Intrinsic Apoptotic Pathway
PI3K/mTOR/AKT Signaling Pathway
NF-κB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
The Retinoblastoma Gene
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...