High-density EEG recordings of the freely moving mice using polyimide-based microelectrode

Area of Science:

• Neuroscience
• Bioengineering
• Surgical Techniques

Background:

• Electroencephalogram (EEG) measures large-scale neuronal electrical activity, crucial for cognitive neuroscience and neurological diagnostics.
• The precise mechanisms of EEG rhythm generation remain largely unknown.
• Mice offer a valuable genetic resource for investigating complex EEG generation processes.

Purpose of the Study:

• To detail the preparation and surgical implantation of a nanofabricated polyimide-based microelectrode (PBM-array) on a mouse skull.
• To provide practical handling and surgical tips for researchers using PBM-arrays for high-resolution mouse EEG.
• To facilitate large-scale brain activity recording in transgenic mice for identifying neural correlates of EEG rhythms and behavior.

Main Methods:

• Step-by-step surgical procedure for PBM-array implantation on the mouse skull.
• Demonstration of techniques for handling the ultra-thin and bifurcated PBM-array.
• Guidance on successful implantation to enable high-resolution EEG acquisition.

Main Results:

• Successful demonstration of PBM-array preparation and implantation techniques.
• Provision of practical tips to overcome challenges associated with handling and implanting the delicate PBM-array.
• Establishment of a method for large-scale brain activity recording in mice.

Conclusions:

• The presented video offers a valuable resource for researchers aiming to utilize PBM-arrays for advanced mouse EEG studies.
• Mastering the described techniques is essential for successful implantation and high-quality data acquisition.
• This method supports the investigation of neurophysiological questions related to EEG generation using genetically modified mouse models.