Related Experiment Video
Updated: Jun 5, 2026
High-throughput Detection Method for Influenza Virus
Published on: February 4, 2012
Quantification of the anti-influenza drug zanamivir in plasma using high-throughput HILIC-MS/MS
Niklas Lindegardh1, Warunee Hanpithakpong, Benjamas Kamanikom
1Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand. niklas@tropmedres.ac
Background:
parenteral zanamivir is a promising drug for the treatment of severe influenza. However, quantification of this polar drug in biological matrices has traditionally been difficult and the methods developed have been relatively insensitive.
Results:
a high-throughput bioanalytical method for the analysis of zanamivir in human plasma using SPE in the 96-well plate format and LC coupled to positive MS/MS has been developed and validated according to US FDA guidelines. The method uses 50 microl of plasma and covers a large working range from 1-50, 000 ng/ml with a LOD of 0.50 ng/ml.
Conclusion:
this new LC-MS/MS assay is more sensitive than previous methods despite using a small plasma volume sample. It is particularly suitable for clinical studies on both parenteral and inhaled zanamivir.
