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Published on: June 10, 2016
[Effects of TGF-β1 on gene expression of connective tissue growth factor in lung fibroblasts]
Jian-Hua Fu1, Hai-Ping Yang, Li Pan
1Department of Pediatrics, Shengjing Hospital, China Medical University, Shenyang 110004, China. fujh@sj-hospital.org
Objective:
To study the effects of transforming growth factor-β1 (TGF-β1) on the gene expression of connective tissue growth factor (CTGF) in cultured lung fibroblasts of embryonic rats in vitro.
Methods:
Wistar rats of embryonic 19 days were used for primary culture of lung fibroblasts (LFs). The cells in the experimental group were treated by different concentrations (1, 5 or 10 ng/mL) and different durations (12, 24 or 48 hrs) of TGF-β1 to stimulate the LFs. The cells in the control group were cultured in serum-free medium. RT-PCR method was applied to detect CTGF mRNA expression in LFs.
Results:
Compared with the control group, the levels of CTGF mRNA in LFs in the experimental group increased significantly (P<0.05). CTGF mRNA expression gradually increased with increasing concentration and duration of TGF-β1 treatment (P<0.05).
Conclusions:
TGF-β1 can stimulate CTGF gene expression in LFs and increase CTGF gene expression in a dose-and time-dependent manner.
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