Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

cDNA clones encoding immunoglobulin lambda chains from rabbit expressing the phenotype c7.

D J Hayzer1, G O Young-Cooper, R G Mage

  • 1Department of Medical Biochemistry, Medical Center of the University of Geneva, Switzerland.

European Journal of Immunology
|December 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Strategies for the design of novel thrombolytic and antithrombolytic agents.

Trends in cardiovascular medicine·2011
Same author

The Kit-activating mutation D816V enhances stem cell factor--dependent chemotaxis.

Blood·2001
Same author

Declawing of neonatal rabbits destined for use in animal biosafety level 4 containment studies.

Contemporary topics in laboratory animal science·2001
Same author

Generation of heterogeneous rabbit anti-DNP antibodies by gene conversion and hypermutation of rearranged VL and VH genes during clonal expansion of B cells in splenic germinal centers.

European journal of immunology·2001
Same author

CD5 and other superantigens may select and maintain rabbit self-renewing B-lymphocytes and human B-CLL cells.

Current topics in microbiology and immunology·2000
Same author

Elvin A. Kabat (1914-2000).

Nature·2000
Same journal

Fast Generation of F(Ab')<sub>2</sub> Fragments From Human IgG Using Fc-Fused IgG-Degrading Enzyme.

European journal of immunology·2026
Same journal

Generation of Regulatory T Cells Against Islet Neoantigen.

European journal of immunology·2026
Same journal

Complement Inhibition in the Clinic: Are We Doing Enough to Protect Patients From Infection?

European journal of immunology·2026
Same journal

Special Issue: yEFIS 3rd Symposium.

European journal of immunology·2026
Same journal

CRISPR/Cas9-Mediated Gene Knockout Reveals a Nonredundant Role for p16<sup>INK4A</sup> in Controlling TCR-Dependent and Independent CD8 T Cell Expansion.

European journal of immunology·2026
Same journal

Induction of Humoral and Cellular Immunity After SARS-CoV-2 JN.1 Vaccination in Individuals With and Without Prior Infection.

European journal of immunology·2026
See all related articles

Rabbit immunoglobulin lambda light chains with c7 and c21 markers represent distinct isotypic forms. Gene C lambda 6 encodes the constant region of c7 lambda chains, differing from the c21-encoding gene C lambda 5.

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Rabbit immunoglobulin lambda light chains exhibit distinct phenotypes, including c7 and c21.
  • Understanding the genetic basis of these isotypic forms is crucial for B cell receptor diversity.

Purpose of the Study:

  • To investigate the genetic basis of rabbit immunoglobulin lambda light chain c7 and c21 phenotypes.
  • To identify the specific genes encoding the constant regions of these lambda chains.

Main Methods:

  • Screening of a rabbit spleen cDNA library using V lambda and C lambda probes.
  • Nucleotide sequencing of hybridizing clones to determine V lambda, J lambda, and C lambda regions.
  • Comparative analysis of gene sequences and Southern blotting.

Related Experiment Videos

Main Results:

  • Three hybridizing clones showed identical V lambda, J lambda, and C lambda regions.
  • The V lambda region demonstrated 97% similarity to the germ-line gene V lambda 2.
  • The C lambda region was identical to the newly identified gene C lambda 6, which differs from C lambda 5 (encoding c21).

Conclusions:

  • Gene C lambda 6 encodes the constant region of c7 lambda chains.
  • The c7 and c21 markers designate two distinct isotypic forms of rabbit lambda light chains.
  • A model is proposed to explain the genetic basis of c7 and c21 phenotypes based on Southern blotting and restriction mapping.