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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

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Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
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A superfluorescent fluorenyl probe with efficient two-photon absorption.

Kevin D Belfield1, Mykhailo V Bondar, Florencio E Hernandez

  • 1Department of Chemistry, University of Central Florida, P.O. Box 162366, Orlando, FL 32816-2366, USA. belfield@mail.ucf.edu

Physical Chemistry Chemical Physics : PCCP
|January 22, 2011
PubMed
Summary
This summary is machine-generated.

This study investigates the photophysical properties of a novel fluorene-based compound (1) for bioimaging. The material exhibits strong fluorescence and efficient two-photon absorption, demonstrating potential for cellular imaging applications.

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Area of Science:

  • Photophysics and photochemistry
  • Organic electronics
  • Biomedical imaging

Background:

  • Fluorene derivatives are widely explored for their optoelectronic properties.
  • Developing novel fluorescent probes for biological imaging is crucial.
  • Understanding excited-state dynamics is key to designing advanced materials.

Purpose of the Study:

  • To investigate the linear photophysical, excited-state absorption (ESA), superfluorescence, and two-photon absorption (2PA) properties of a novel fluorene-based compound (1).
  • To evaluate the potential application of compound (1) in biological imaging.
  • To characterize the absorption and emission behavior in various media.

Main Methods:

  • Steady-state and time-resolved fluorescence spectroscopy.
  • Excited-state absorption (ESA) spectroscopy.
  • Degenerate two-photon absorption (2PA) spectroscopy using femtosecond laser excitation.
  • In vitro fluorescence imaging of HCT 116 cells.

Main Results:

  • Compound (1) displays complex one-photon absorption, strong solvatochromic effects, and high fluorescence quantum yields (≈1.0) in organic solvents.
  • ESA spectra suggest potential for light amplification, and efficient superfluorescence was observed in cyclohexane.
  • Two distinct 2PA bands were identified, with maximum 2PA cross sections up to 1700 GM.
  • Successful one- and two-photon fluorescence imaging of HCT 116 cells was achieved.

Conclusions:

  • Compound (1) possesses excellent photophysical properties, including high fluorescence efficiency and significant two-photon absorption cross-sections.
  • The demonstrated in vitro cell imaging capabilities highlight its promise as a fluorescent probe for biological applications.
  • Further research into its photostability and in vivo performance is warranted.