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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 5, 2026

Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus

Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus

Published on: November 6, 2018

PALM and STORM: unlocking live-cell super-resolution.

Ricardo Henriques¹, Caron Griffiths, E Hesper Rego

¹Unidade de Biofisica e Expressão Genetica, Instituto de Medicina Molecular, Faculdade de Medicina Universidade de Lisboa, Lisboa, Portugal. rhenriques@fm.ul.pt

|January 22, 2011

Summary

Super-resolution microscopy now enables visualization of living cells at the molecular level. Recent advances in single molecule localization microscopy methods like PALM and STORM are key to this breakthrough.

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Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)

Published on: December 1, 2016

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Last Updated: Jun 5, 2026

Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus

Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus

Published on: November 6, 2018

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

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Published on: June 24, 2017

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)

Published on: December 1, 2016

Area of Science:

Cellular Biology
Biophysics
Microscopy

Background:

Live-cell fluorescence light microscopy is crucial for studying cellular processes.
Super-resolution imaging achieves nanometer-scale molecular visualization, rivaling electron microscopy.
Integrating live-cell imaging with super-resolution has been a significant challenge.

Purpose of the Study:

To review recent advancements in super-resolution imaging of living cells.
To highlight breakthroughs enabling live-cell super-resolution microscopy.
To discuss the potential for observing dynamic single-molecule interactions in real-time.

Main Methods:

Focuses on single molecule localization microscopy (SMLM) techniques.
Reviews developments in Photoactivated Localization Microscopy (PALM).
Examines advances in Stochastic Optical Reconstruction Microscopy (STORM).

Main Results:

Super-resolution microscopy can now be applied to live-cell imaging.
PALM and STORM methods have been refined for live-cell applications.
Achieved resolutions previously limited to fixed samples or electron microscopy.

Conclusions:

Live-cell super-resolution microscopy is now a reality.
These advancements unlock new possibilities for studying dynamic biological processes at the molecular level.
Future research can leverage these techniques to explore single-molecule dynamics in living systems.