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Updated: Jun 5, 2026

Isolation of Sertoli Cells and Peritubular Cells from Rat Testes

Isolation of Sertoli Cells and Peritubular Cells from Rat Testes

Published on: February 8, 2016

Urocortins are present in the rat testis.

Soon Lee¹, Brian Braden, Sang Soo Kang

¹The Clayton Foundation Laboratories for Peptide Biology, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.

|January 25, 2011

Summary

Testosterone synthesis is influenced by testicular factors. This study reveals that urocortins (Ucns), not corticotropin-releasing factor (CRF), are present in rat testes and their levels increase with alcohol exposure.

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Area of Science:

Endocrinology
Reproductive Biology
Molecular Biology

Background:

Testosterone (T) synthesis relies on luteinizing hormone (LH) and poorly understood testicular factors.
Previous reports suggested corticotropin-releasing factor (CRF) inhibits T secretion in rat testes.
Urocortins (Ucns) cross-react with CRF reagents, casting doubt on prior findings.

Purpose of the Study:

To investigate the presence and role of CRF-like peptides in adult male rat testes.
To determine if alcohol exposure affects testicular CRF-like peptide levels.

Main Methods:

Quantitative real-time PCR (qRT-PCR) and microarray analysis to detect mRNA expression.
Immunohistochemistry to localize peptide expression.
Alcohol exposure model in rats.

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Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions

Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions

Published on: March 12, 2019

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Last Updated: Jun 5, 2026

Isolation of Sertoli Cells and Peritubular Cells from Rat Testes

Isolation of Sertoli Cells and Peritubular Cells from Rat Testes

Published on: February 8, 2016

Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions

Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions

Published on: March 12, 2019

Main Results:

CRF was barely detectable in rat testes, unlike hypothalami.
Strong signals for Ucn 1 mRNA were found in testes, with weaker Ucn 2 and 3 signals.
Ucn 1 mRNA expression in Leydig cells increased significantly after alcohol administration.

Conclusions:

Urocortins, particularly Ucn 1, are the predominant CRF-related peptides in adult male rat testes.
Alcohol exposure elevates testicular Ucn 1 mRNA levels.
These findings clarify the presence of CRF-like peptides in the male gonads.