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Updated: Jun 5, 2026
Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation
Published on: July 31, 2015
Improved GFP gene transfection mediated by polyamidoamine dendrimer-functionalized multi-walled carbon nanotubes with
Weiling Qin1, Keqin Yang, Hao Tang
1Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, PR China.
Abstract:
The multi-walled carbon nanotubes (MWCNTs)-polyamidoamine (PAMAM) hybrid was prepared by covalent linkage approach, and characterized by transmission electron microscopy, Fourier transform infrared spectroscopy and ultraviolet-visible spectrometry. The PAMAM dendrimers were present on the surface of MWCNTs in high density, and the MWCNT-PAMAM hybrid exhibited good dispersibility and stability in aqueous solution. The interaction between MWCNT-PAMAM with plasmid DNA of enhanced green fluorescence protein (pEGFP-N1), intracellular trafficking of the hybrid, transfection performance and cytotoxicity to HeLa cells were evaluated in detail. We found that the MWCNT-PAMAM hybrid possessed good pEGFP-N1 immobilization ability and could efficiently delivery GFP gene into cultured HeLa cells. The surface modification of MWCNTs with PAMAM improved the transfection efficiency 2.4 and 0.9 times, and simultaneously decreased cytotoxicity by about 38%, as compared with mixed acid-treated MWCNTs and pure PAMAM dendrimers. The MWCNT-PAMAM hybrid can be considered as a new carrier for the delivery of biomolecules into mammalian cells. Therefore, this novel system may have good potential applications in biology and therapy, including gene delivery systems.
