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Updated: Jun 5, 2026
A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
Published on: March 9, 2016
Sensitive counting of viable Enterobacteriaceae in seawaters and relationship with fecal indicators
J Baudart1, A Robyns, S Peuchet
1UPMC Univ Paris 06, UMR 7621, LOMIC, Observatoire Océanologique, F-66650, Banyuls/mer, France. baudart@obs-banyuls.fr
Abstract:
A fluorescent in situ hybridization based assay was used to enumerate viable Enterobacteriaceae members in seawaters by solid phase cytometry. The method was specific, highly sensitive (1cell/100ml) and allowed the quantification of VNC Enterobacteriaceae cells during an osmotic stress. Investigations on contaminated coastal seawater revealed a strong correlation between Enterobacteriaceae counts and standard fecal indicators.
