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Updated: Jun 5, 2026

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
Published on: November 2, 2009
Micropilot: automation of fluorescence microscopy-based imaging for systems biology
Christian Conrad1, Annelie Wünsche, Tze Heng Tan
1Advanced Light Microscopy Facility, European Molecular Biology Laboratory, Heidelberg, Germany.
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Quantitative microscopy relies on imaging of large cell numbers but is often hampered by time-consuming manual selection of specific cells. The 'Micropilot' software automatically detects cells of interest and launches complex imaging experiments including three-dimensional multicolor time-lapse or fluorescence recovery after photobleaching in live cells. In three independent experimental setups this allowed us to statistically analyze biological processes in detail and is thus a powerful tool for systems biology.
