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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
X-ray Imaging01:24

X-ray Imaging

German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with X-rays, and by 1900, X-ray was widely...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
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Improved detection limits using a hand-held optical imager with coregistration capabilities.

Sarah J Erickson1, Sergio L Martinez, Jean Gonzalez

  • 1Department of Biomedical Engineering, Florida International University, 10555 West Flagler Street EC2610; Miami, FL 33174, USA.

Biomedical Optics Express
|January 25, 2011
PubMed
Summary
This summary is machine-generated.

Optical imaging offers a non-invasive approach for breast cancer detection. Summing multiple scans enhances fluorescence imaging, enabling deeper detection of cancerous targets within tissue phantoms.

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Area of Science:

  • Biomedical optics
  • Medical imaging
  • Cancer diagnostics

Background:

  • Optical imaging is a promising non-invasive, non-ionizing technique for breast cancer diagnosis.
  • A novel hand-held optical imager with coregistration capabilities has been developed for flexible, real-time imaging.
  • The imager accommodates various tissue geometries and curvatures.

Purpose of the Study:

  • To evaluate the efficacy of fluorescence-enhanced optical imaging for deeper target detection.
  • To assess performance under both ideal and challenging (100:1 contrast) uptake conditions.
  • To validate findings in liquid tissue phantoms and in vitro settings.

Main Methods:

  • Utilized a hand-held optical imager with coregistration.
  • Conducted fluorescence-enhanced optical imaging experiments.
  • Employed liquid tissue phantoms and in vitro models.
  • Acquired single and multiple fluorescence intensity images (scans).

Main Results:

  • Fluorescent targets were successfully detected at greater depths by summing multiple scans compared to single scans.
  • Deeper detection was demonstrated under both perfect and imperfect (100:1 contrast) uptake conditions.
  • The developed imaging system showed capability for flexible imaging of different tissue volumes.

Conclusions:

  • Summation of multiple fluorescence intensity images significantly improves the detection depth of targets in optical imaging.
  • This technique holds potential for enhanced non-invasive breast cancer diagnosis.
  • The developed optical imager is suitable for flexible and deeper tissue imaging.