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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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Related Experiment Video

Updated: Jun 5, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
08:31

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

High-speed focal modulation microscopy using acousto-optical modulators.

Shau Poh Chong, Chee Howe Wong, Kit Fei Wong

    Biomedical Optics Express
    |January 25, 2011
    PubMed
    Summary
    This summary is machine-generated.

    Focal Modulation Microscopy (FMM) uses acousto-optic modulators for MHz-speed laser modulation, enabling real-time deep tissue imaging by reducing background fluorescence. This advanced technique visualizes fine structures in biological samples effectively.

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    Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
    09:57

    Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

    Published on: July 25, 2022

    Area of Science:

    • Biomedical Optics
    • Microscopy Techniques
    • Fluorescence Imaging

    Background:

    • Imaging deep within biological tissues is challenging due to out-of-focus fluorescence.
    • Conventional microscopy techniques struggle to reject this background noise.
    • Need for advanced imaging methods for real-time visualization of biological structures.

    Purpose of the Study:

    • To implement and demonstrate a high-speed Focal Modulation Microscopy (FMM) system.
    • To enhance FMM by utilizing acousto-optic modulators (AOMs) for rapid laser modulation.
    • To enable real-time fluorescence imaging deep inside biological tissues.

    Main Methods:

    • Implemented FMM using acousto-optic modulators (AOMs) for laser intensity modulation.
    • Achieved MHz-range modulation speeds for enhanced imaging performance.
    • Utilized single-photon excitation for fluorescence microscopy.

    Main Results:

    • Demonstrated real-time image acquisition capabilities of the enhanced FMM system.
    • Successfully imaged fluorescence-labeled vasculatures in mouse brain tissue.
    • Validated the technique using a self-made tissue phantom.

    Conclusions:

    • High-speed FMM with AOMs effectively reduces out-of-focus fluorescence background.
    • The implemented system enables real-time imaging of biological structures deep within tissues.
    • FMM is a powerful technique for advanced biomedical imaging applications.