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Related Concept Videos

Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Structural Organization of the Human Body: An Overview01:18

Structural Organization of the Human Body: An Overview

It is convenient to consider the body's structures in terms of fundamental levels of organization that increase in complexity: subatomic particles, atoms, molecules, organelles, cells, tissues, organs, organ systems, and organisms.
To study the chemical level of organization, scientists consider the simplest building blocks of matter: subatomic particles, atoms, and molecules. All matter in the universe is composed of one or more unique pure substances called elements, familiar examples of...
Levels of Organization01:09

Levels of Organization

Biological organization is the classification of biological structures, ranging from atoms at the bottom of the hierarchy to the Earth's biosphere. Each level of the hierarchy represents an increase in complexity that builds upon the previous level.Molecules Are Composed of Atoms, and Biomolecules Are Assembled from Molecules:The most basic levels include atoms, molecules, and biomolecules. Atoms, the smallest unit of ordinary matter, are composed of a nucleus and electrons. Molecules comprise...
Eukaryotic Compartmentalization01:46

Eukaryotic Compartmentalization

One of the distinguishing features of eukaryotic cells is that they contain membrane-bound organelles, such as the nucleus and mitochondria, that carry out specialized functions. Since biological membranes are only selectively permeable to solutes, they help create a compartment with controlled conditions inside an organelle. These microenvironments are tailored to the organelle's specific functions and help isolate them from the surrounding cytosol.
For example, lysosomes in the animal cells...
Eukaryotic Compartmentalization01:37

Eukaryotic Compartmentalization

One of the distinguishing features of eukaryotic cells is that they contain membrane-bound organelles, such as the nucleus and mitochondria, that carry out specialized functions. Since biological membranes are only selectively permeable to solutes, they help create a compartment with controlled conditions inside an organelle. These microenvironments are tailored to the organelle's specific functions and help isolate them from the surrounding cytosol.
For example, lysosomes in the animal cells...

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Related Experiment Video

Updated: Jun 5, 2026

The Organoid Reconstitution Assay (ORA) for the Functional Analysis of Intestinal Stem and Niche Cells
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The Organoid Reconstitution Assay (ORA) for the Functional Analysis of Intestinal Stem and Niche Cells

Published on: November 20, 2017

State of the APC/C: organization, function, and structure.

Janel R McLean1, Denis Chaix, Melanie D Ohi

  • 1Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.

Critical Reviews in Biochemistry and Molecular Biology
|January 26, 2011
PubMed
Summary

The anaphase-promoting complex/cyclosome (APC/C) is a large E3 ubiquitin ligase crucial for cell cycle regulation. Recent structural studies, combined with genetic and biochemical data, illuminate its complex regulation, substrate recognition, and catalytic mechanisms.

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Purification and Aggregation of the Amyloid Precursor Protein Intracellular Domain
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Purification and Aggregation of the Amyloid Precursor Protein Intracellular Domain

Published on: August 28, 2012

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Last Updated: Jun 5, 2026

The Organoid Reconstitution Assay (ORA) for the Functional Analysis of Intestinal Stem and Niche Cells
09:38

The Organoid Reconstitution Assay (ORA) for the Functional Analysis of Intestinal Stem and Niche Cells

Published on: November 20, 2017

Purification and Aggregation of the Amyloid Precursor Protein Intracellular Domain
10:08

Purification and Aggregation of the Amyloid Precursor Protein Intracellular Domain

Published on: August 28, 2012

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • The ubiquitin-proteasome system regulates essential cellular processes.
  • The anaphase-promoting complex/cyclosome (APC/C) is a large E3 ubiquitin ligase vital for cell cycle progression.
  • APC/C's complexity has hindered high-resolution structural analysis.

Purpose of the Study:

  • To review recent advancements in understanding the APC/C.
  • To discuss the APC/C's structure, substrate recognition, and catalysis.
  • To integrate structural data with genetic and biochemical findings.

Main Methods:

  • Single particle electron microscopy (EM) for lower-resolution structures.
  • Integration of existing genetic and biochemical data.
  • Review of recent literature on APC/C regulation and function.

Main Results:

  • Lower-resolution APC/C structures have been determined using EM.
  • These structures provide insights into APC/C regulation when combined with other data.
  • Progress has been made in understanding substrate recognition and catalysis.

Conclusions:

  • Despite its size and complexity, structural studies are revealing APC/C mechanisms.
  • Understanding APC/C regulation is key to comprehending cell cycle control.
  • Further research will continue to elucidate APC/C's role in cellular processes.