Related Experiment Video
Updated: Jun 5, 2026
10:19
Evaluation of Biomaterials for Bladder Augmentation using Cystometric Analyses in Various Rodent Models
Published on: August 9, 2012
Pharmacologic characteristics of bladder micturition function in anesthetized mice
Jian Zhou1, Guo-Dong Luan, Lei-Ming Ren
1Department of Pharmacology, School of Pharmacy Hebei Medical University, Shijiazhuang, People's Republic of China.
Comparative Medicine
|January 26, 2011
Summary
This study investigated how different receptor agonists and antagonists affect mouse bladder function. Findings show that muscarinic, alpha-1, and beta-adrenoceptor activation significantly alters bladder pressure and voiding patterns.
Area of Science:
- Pharmacology
- Urology
- Physiology
Background:
- Bladder micturition function is regulated by complex neural and hormonal pathways.
- Adrenoceptors and cholinoceptors play crucial roles in modulating bladder activity and blood pressure.
- Understanding these receptor interactions is vital for developing targeted therapies for urinary dysfunction.
Purpose of the Study:
- To investigate the effects of specific adrenoceptor and cholinoceptor agonists and antagonists on bladder micturition function in anesthetized mice.
- To elucidate the distinct roles of α(1)-adrenoceptors, β-adrenoceptors, and muscarinic cholinoceptors in regulating bladder pressure and voiding dynamics.
- To assess the impact of these agents on concurrent blood pressure changes.
Main Methods:
- Administration of phenylephrine (α(1)-agonist), isoprenaline (β-agonist), carbachol (muscarinic agonist), and doxazosin (α(1)-antagonist) to anesthetized mice.
- Measurement of bladder pressure (vesical micturition pressure, basal pressure, intercontraction interval) and blood pressure using a data acquisition system.
- Dose-dependent administration of agents to observe graded responses.
Main Results:
- Phenylephrine dose-dependently increased vesical micturition pressure.
- Carbachol dose-dependently increased the intercontraction interval and micturition time.
- Isoprenaline dose-dependently decreased vesical micturition pressure and increased micturition time.
- Doxazosin did not affect bladder function but inhibited phenylephrine-induced pressure increases.
- Carbachol and isoprenaline caused transient blood pressure falls; doxazosin induced a prolonged hypotensive effect; phenylephrine transiently increased blood pressure.
Conclusions:
- Activation of muscarinic cholinoceptors decreases voiding frequency and increases bladder capacity.
- α(1)-adrenoceptor activation primarily increases vesical micturition pressure.
- β-adrenoceptor activation decreases vesical micturition pressure and prolongs micturition time.
Related Concept Videos
01:26The Micturition Reflex
Urination, or micturition involves the coordination of the bladder's detrusor muscle and two sphincters to ensure controlled bladder emptying.
The process begins with bladder filling, where the bladder wall stretches as urine accumulates. This stretching activates the urine storage reflex, mediated by the sacral spinal segments and the pontine storage center. Efferent sympathetic impulses stimulate the detrusor muscle to relax and the internal urethral sphincter to contract, facilitating urine...
The process begins with bladder filling, where the bladder wall stretches as urine accumulates. This stretching activates the urine storage reflex, mediated by the sacral spinal segments and the pontine storage center. Efferent sympathetic impulses stimulate the detrusor muscle to relax and the internal urethral sphincter to contract, facilitating urine...
01:28Cholinergic Antagonists: Pharmacological Actions
Antimuscarinic drugs block muscarinic receptors in multiple systems, including the gut, eye, smooth muscles, respiratory tract, cardiovascular, and central nervous systems. They produce similar effects with varying selectivity depending on the specific agent and tissue. Here are the key pharmacological actions of antimuscarinics:
Gastrointestinal Effects: Antimuscarinics reduce gut contractions, increase gastric emptying, and slow intestinal transit. They partly inhibit gastric acid secretion...
Gastrointestinal Effects: Antimuscarinics reduce gut contractions, increase gastric emptying, and slow intestinal transit. They partly inhibit gastric acid secretion...