Spatially-chirped modulation imaging of absorbtion and fluorescent objects on single-element optical detector
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Updated: Jun 5, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
Published on: December 9, 2013
Greg Futia1, Philip Schlup, David G Winters
1Department of Electrical and Computer Engineering, Colorado State University, Fort Collins, Colorado 80523, USA.
Abstract:
Line imaging of fluorescent and absorptive objects with a single-pixel imaging technique that acquires one-dimensional cross-sections through a sample by imposing a spatially-varying amplitude modulation on the probing beam is demonstrated. The fluorophore concentration or absorber distribution of the sample is directly mapped to modulation frequency components of the spatially-integrated temporal signal. Time-domain signals are obtained from a single photodiode, with object spatial frequency correlation encoded in time-domain bursts in the electronic signal from the photodiode.
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