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Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
Photoluminescence: Fluorescence and Phosphorescence01:23

Photoluminescence: Fluorescence and Phosphorescence

Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
08:31

Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells

Published on: September 16, 2014

Molecular decoding using luminescence from an entangled porous framework.

Yohei Takashima1, Virginia Martínez Martínez, Shuhei Furukawa

  • 11] ERATO Kitagawa Integrated Pores Project, Japan Science and Technology Agency (JST), Kyoto Research Park Building no. 3, Shimogyo-ku, Kyoto 600-8815, Japan. [2] Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.

Nature Communications
|January 27, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces a novel molecular decoding strategy using an entangled porous framework. This framework distinguishes between different aromatic compounds, enabling multicolor luminescence for precise chemical sensing.

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Lensless Fluorescent Microscopy on a Chip
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Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
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11:23

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Published on: August 17, 2011

Area of Science:

  • Materials Science
  • Supramolecular Chemistry
  • Chemical Sensing

Background:

  • Traditional chemosensors identify single targets but lack differentiation capabilities among similar molecules.
  • Developing molecular recognition systems that can distinguish between closely related chemical species remains a significant challenge.

Purpose of the Study:

  • To develop a molecular decoding strategy using a single host domain capable of differentiating between a class of molecules.
  • To create a chemoresponsive material that exhibits multicolor luminescence based on the specific chemical substituent of an aromatic guest.

Main Methods:

  • Synthesis of a decoding host by embedding naphthalenediimide into an entangled porous framework with dynamic structural properties.
  • Investigation of the luminescent response of the host upon incorporation of various aromatic compounds.
  • Analysis of the structural transformation and guest-host interactions using spectroscopic methods.

Main Results:

  • An intense turn-on emission was observed upon the incorporation of aromatic compounds.
  • The luminescent color varied depending on the chemical substituent of the aromatic guest, demonstrating molecular differentiation.
  • The observed multicolor luminescence resulted from enhanced naphthalenediimide-aromatic guest interactions driven by an induced-fit structural transformation.
  • A nonlinear sensor response to guest concentration was observed due to cooperative structural transitions.

Conclusions:

  • The developed entangled porous framework acts as a molecular decoder, distinguishing between aromatic compounds through multicolor luminescence.
  • This strategy offers a new approach for designing advanced chemosensors with enhanced specificity and readout capabilities.
  • The induced-fit mechanism in dynamic frameworks provides a powerful platform for molecular recognition and sensing applications.