Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Electrical discrimination of lysine methylation states at the single-molecule level.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2026
Same author

Nanofluidic systems for ionic intelligence.

Nanoscale horizons·2026
Same author

Three-dimensional plasmonic nanopores for DNA-PAINT and dual-material Au/Si architectures.

Journal of nanobiotechnology·2026
Same author

Single-molecule detection of amino acid phosphorylation using electron tunnelling currents: toward neurodegenerative disease diagnosis.

Nanoscale·2026
Same author

Chemistry-driven autonomous nanopore membranes.

Nature communications·2026
Same author

Beyond ensemble averages: single-entity approaches for complex systems.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2026

Related Experiment Video

Updated: Jun 5, 2026

High Resolution Physical Characterization of Single Metallic Nanoparticles
09:56

High Resolution Physical Characterization of Single Metallic Nanoparticles

Published on: June 28, 2019

Single-molecule identification via electric current noise.

Makusu Tsutsui1, Masateru Taniguchi, Tomoji Kawai

  • 1The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.

Nature Communications
|January 27, 2011
PubMed
Summary
This summary is machine-generated.

Researchers identified single organic molecules by observing quantum-fluctuation-induced inelastic noise. This noise, arising from molecular vibrations and electron tunneling, provides a unique molecular signature for detection.

More Related Videos

All-electronic Nanosecond-resolved Scanning Tunneling Microscopy: Facilitating the Investigation of Single Dopant Charge Dynamics
11:33

All-electronic Nanosecond-resolved Scanning Tunneling Microscopy: Facilitating the Investigation of Single Dopant Charge Dynamics

Published on: January 19, 2018

Electricity-Free, Sequential Nucleic Acid and Protein Isolation
09:52

Electricity-Free, Sequential Nucleic Acid and Protein Isolation

Published on: May 15, 2012

Related Experiment Videos

Last Updated: Jun 5, 2026

High Resolution Physical Characterization of Single Metallic Nanoparticles
09:56

High Resolution Physical Characterization of Single Metallic Nanoparticles

Published on: June 28, 2019

All-electronic Nanosecond-resolved Scanning Tunneling Microscopy: Facilitating the Investigation of Single Dopant Charge Dynamics
11:33

All-electronic Nanosecond-resolved Scanning Tunneling Microscopy: Facilitating the Investigation of Single Dopant Charge Dynamics

Published on: January 19, 2018

Electricity-Free, Sequential Nucleic Acid and Protein Isolation
09:52

Electricity-Free, Sequential Nucleic Acid and Protein Isolation

Published on: May 15, 2012

Area of Science:

  • Physical Chemistry
  • Molecular Electronics
  • Nanotechnology

Background:

  • Label-free, real-time single-molecule detection is crucial for high-throughput biosensing.
  • Molecular fluctuations (noise) often obscure molecular identity, hindering detection.

Purpose of the Study:

  • To demonstrate molecular identification using quantum-fluctuation-induced inelastic noise in single organic molecules.
  • To explore the potential of inelastic noise as a molecular signature.

Main Methods:

  • Investigated current fluctuations through single organic molecules connected to two electrodes.
  • Analyzed oscillations synchronous to electric field excitations of molecular vibrational modes.

Main Results:

  • Observed increased current oscillations linked to inelastic electron tunneling.
  • Demonstrated that charge interaction with nuclear dynamics significantly impacts noise properties in single-molecule junctions.

Conclusions:

  • Inelastic noise can serve as a valuable molecular signature for single-molecule identification.
  • This approach offers a new pathway for label-free, real-time molecular detection.