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Published on: September 8, 2023
Developing a mouse model of acute bacterial rhinosinusitis
Mingzhu Jin1, Zhaowei Gu, Zhigang Bian
1Department of Otolaryngology, Shengjing Hospital of China Medical University, Sanhao Street 36, Shenyang, Liaoning, China. jinmingzhu1984@sohu.com
Abstract:
The objective of this study is to establish a mouse model of acute bacterial rhinosinusitis. 179 healthy male BALB/c mice were divided into four groups in this randomized and controlled study. Sponge slivers impregnated with methicillin-resistant Staphylococcus aureus (MRSA COL) suspension were inserted into the right nasal cavities for group A; sponge slivers impregnated with sterile saline were inserted into the right nasal cavities for group B; group C mice were inoculated with MRSA COL suspension in right nasal cavities; group D was control group without any treatment. Mice were killed on days 1, 4, 7 and 14, respectively. Nasal lavage fluid was prepared for microbiological culture. Histological examinations of nasal specimens were performed to observe the severity of inflammatory reaction. Acute bacterial rhinosinusitis was induced in all group A mice. Less severe inflammation was seen in partial group B mice compared with that in group A mice (P ≤ 0.05). No inflammatory reaction was found in group C and D mice. In conclusion, a mouse model of acute bacterial rhinosinusitis has been developed successfully using an easier, less invasive and potentially more reversible technique than those used in previous studies.
Insights
Researchers developed a new mouse model for acute bacterial rhinosinusitis using methicillin-resistant Staphylococcus aureus (MRSA). This less invasive method successfully induced rhinosinusitis, offering a valuable tool for studying the condition.
Area of Science:
- Otorhinolaryngology
- Infectious Diseases
- Animal Models
Background:
- Acute bacterial rhinosinusitis (ABRS) is a common condition requiring effective research models.
- Existing models for studying ABRS in mice often involve invasive procedures.
Purpose of the Study:
- To establish a novel, less invasive mouse model for acute bacterial rhinosinusitis.
- To utilize methicillin-resistant Staphylococcus aureus (MRSA) for inducing the condition in a controlled manner.
Main Methods:
- 179 BALB/c mice were divided into four groups: MRSA-impregnated sponges, sterile saline sponges, direct MRSA inoculation, and untreated control.
- Mice were euthanized on days 1, 4, 7, and 14 post-inoculation.
- Nasal lavage and histological examination were performed to assess inflammation and bacterial presence.
Main Results:
- Successful induction of acute bacterial rhinosinusitis in mice treated with MRSA-impregnated sponges.
- Partial, less severe inflammation observed in mice receiving sterile saline sponges compared to the MRSA sponge group (P ≤ 0.05).
- No significant inflammatory reactions were noted in the direct inoculation or control groups.
Conclusions:
- A successful and less invasive mouse model for acute bacterial rhinosinusitis was developed.
- The MRSA-impregnated sponge technique offers a potentially more reversible method for ABRS research.
- This model provides a valuable platform for investigating ABRS pathogenesis and therapeutic strategies.
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