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Updated: Jun 4, 2026

Isolation and Cultivation of Mandibular Bone Marrow Mesenchymal Stem Cells in Rats
Published on: August 25, 2020
[Effect of bone-marrow mesenchymal stem cells on the immune function of aging rats]
Zhi-hong Wang1, Kun-yuan Guo, Yu-lian Yang
1Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. zhihongwang1980@126.com
Objective:
To investigate the effect of transplantation of bone-marrow mesenchymal stem cells (MSCs) on the immune functions of aging rats.
Methods:
Healthy SD rats were randomized into normal control, aging model group and MSCs group. The aging model was established by daily subcutaneous injection of D-galactose for 4 consecutive months. MSCs were isolated from the bone marrow of adult SD rats and injected (3×10(6) MSCs) in rats in the MSCs group via the tail vein once a week for 4 weeks. The spleen index, activity of T lymphocytes and the levels of IL-2 and IL-10 in spleen were measured, and the pathological changes of the spleen were observed after the treatments.
Results:
MSCs transplantation enhanced the cellular immune function of aging rats manifested by obviously increased spleen index, activity of T lymphocyte and the level of IL-2, and lowered level of IL-10 in the spleen. The rats in the aging model group showed serious spleen injury, which was obviously lessened by MSCs injection.
Conclusion:
MSCs transplantation can improve the cellular immune function of aging rats and ameliorate spleen injury induced by D-galactose.
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