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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Unexpected role of nucleoporins in coordination of cell cycle progression

Hiroshi Nakano1, Wei Wang, Chieko Hashizume

  • 1Frontier Science Organization, Kanazawa University, Kanazawa, Japan.

Cell Cycle (Georgetown, Tex.)
|January 29, 2011
PubMed

Insights

Nuclear pore proteins (nucleoporins/Nups) are crucial for cell division. Altered Nup levels can lead to aneuploidy, a hallmark of many human cancers, highlighting their role in mitosis.

Area of Science:

  • Cell Biology
  • Cancer Biology
  • Molecular Biology

Background:

  • Aneuploidy, characterized by abnormal chromosome numbers, is prevalent in human cancers.
  • Nuclear pore proteins (nucleoporins/Nups) regulate nuclear transport and have emerging roles in cell division.

Purpose of the Study:

  • To review the involvement of nucleoporins in mitosis.
  • To discuss the link between nucleoporin concentration and aneuploidy.
  • To explore the potential functions of nucleoporins during cell division.

Main Methods:

  • Literature review of studies investigating nucleoporins and mitosis.
  • Analysis of molecular networks involving nucleoporins in mitotic processes.
  • Discussion of experimental evidence linking nucleoporin alterations to aneuploidy.

Main Results:

  • Nucleoporins participate in key mitotic events: chromosome condensation, sister chromatid cohesion, kinetochore assembly, and spindle formation.
  • Changes in intracellular nucleoporin concentrations are frequently associated with the development of aneuploidy.

Conclusions:

  • Nucleoporins play a significant and multifaceted role in ensuring accurate chromosome segregation during mitosis.
  • Dysregulation of nucleoporin levels represents a potential driver of aneuploidy in cancer development.
  • Further research into nucleoporin functions during mitosis is warranted.

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