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Related Concept Videos

Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Related Experiment Video

Updated: Jun 4, 2026

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation
07:40

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation

Published on: July 31, 2015

Graphene based gene transfection.

Liangzhu Feng1, Shuai Zhang, Zhuang Liu

  • 1Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Institute of Functional Nano & Soft Materials, Soochow University, Suzhou, Jiangsu, China.

Nanoscale
|January 29, 2011
PubMed
Summary
This summary is machine-generated.

Graphene oxide (GO) serves as a novel, non-toxic nano-vehicle for gene delivery. GO-polyethyleneimine (PEI) complexes show reduced toxicity and efficient gene transfection, promising for non-viral gene therapy.

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Last Updated: Jun 4, 2026

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Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Nanotechnology

Background:

  • Graphene is a promising material in biomedicine.
  • Gene transfection requires efficient and non-toxic delivery vectors.
  • Current non-viral vectors often face challenges with toxicity and efficiency.

Purpose of the Study:

  • To investigate graphene oxide (GO) as a nano-vehicle for gene delivery.
  • To evaluate the efficiency and toxicity of GO-polyethyleneimine (PEI) complexes for gene transfection.
  • To compare the performance of GO-PEI complexes with bare PEI.

Main Methods:

  • Graphene oxide (GO) was complexed with polyethyleneimine (PEI) of two molecular weights (1.2 kDa and 10 kDa).
  • Complexes (GO-PEI-1.2k and GO-PEI-10k) were characterized for stability in physiological solutions.
  • Cellular toxicity assays were performed on treated cells.
  • Gene transfection efficiency was assessed using enhanced green fluorescence protein (EGFP) in HeLa cells.

Main Results:

  • GO-PEI complexes were stable in physiological solutions.
  • The GO-PEI-10k complex demonstrated significantly reduced cellular toxicity compared to bare PEI-10k.
  • GO-PEI-1.2k showed high EGFP transfection efficiency, unlike bare PEI-1.2k.
  • GO-PEI-10k exhibited comparable EGFP transfection efficiency to PEI-10k but with lower toxicity.

Conclusions:

  • Graphene oxide is a viable non-toxic nano-vector for efficient gene delivery.
  • GO-PEI complexes offer a promising platform for non-viral gene therapy applications.
  • This approach holds potential for improving the safety and efficacy of gene-based treatments.