Related Experiment Video
Updated: Jun 4, 2026
Controlled Semi-Automated Laser-Induced Injuries for Studying Spinal Cord Regeneration in Zebrafish Larvae
Published on: November 22, 2021
Histological analysis of low-intensity laser therapy effects in peripheral nerve regeneration in Wistar rats
Cibele Nazaré da Silva Câmara1, Marcus Vinicius Henriques Brito, Edvaldo Lima Silveira
1Department of Physiotherapy, UNAMA, Belem, PA, Brazil. camaracibele@yahoo.com.br
Purpose:
Analyze the influence of low-intensity laser therapy in the sciatic nerve regeneration of rats submitted to controlled crush through histological analysis.
Methods:
Were used 20 Wistar rats, to analyze the influence of low-intensity laser therapy in the sciatic nerve regeneration, where the injury of the type axonotmesis was induced by a haemostatic clamp Crile (2nd level of the rack). The animals were randomly distributed in 2 groups. Control group (CG n = 10) and Laser group (LG n = 10). These were subdivided in 2 subgroups each, according to the euthanasia period: (CG14 - n = 5 and CG21 - n = 5) and (LG14 - n = 5 and LG21 - n = 5). At the end of treatment, the samples were removed and prepared for histological analysis, where were analyzed and quantified the following findings: Schwann cells, myelinic axons with large diameter and neurons.
Results:
In the groups submitted to low-intensity laser therapy, were observed an increase in the number of all analyzed aspects with significance level.
Conclusion:
The irradiation with low intensity laser (904nm) influenced positively the regeneration of the sciatic nerve in Wistar rats after being injured by crush (axonotmesis), becoming the nerve recovery more rapid and efficient.
