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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Related Experiment Video

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Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans
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Published on: January 1, 2018

Codon adaptation-based control of protein expression in C. elegans.

Stefanie Redemann1, Siegfried Schloissnig, Susanne Ernst

  • 1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden Germany.

Nature Methods
|February 1, 2011
PubMed
Summary

We developed a method to control protein levels in Caenorhabditis elegans using synthetic genes with adapted codons. This technique allows precise regulation of gene expression for studying developmental processes like spindle force.

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Published on: September 25, 2013

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Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans
07:53

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Published on: January 1, 2018

Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes
18:38

Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes

Published on: September 25, 2013

Area of Science:

  • Developmental Biology
  • Molecular Genetics
  • Biotechnology

Background:

  • Controlling protein levels is crucial for understanding gene function and developmental processes.
  • Native genetic regulation can be complex and difficult to manipulate directly.
  • Synthetic biology offers tools to engineer genetic systems with novel functionalities.

Purpose of the Study:

  • To present a novel method for controlling protein levels in Caenorhabditis elegans under native genetic regulation.
  • To demonstrate the utility of codon-adapted synthetic genes for precise gene expression modulation.
  • To investigate the relationship between G-protein regulator levels and spindle force in C. elegans embryos.

Main Methods:

  • Design and synthesis of codon-adapted genes for Caenorhabditis elegans.
  • Utilizing a web tool (C. elegans codon adapter) for gene design.
  • Measuring protein levels and spindle forces in C. elegans embryos.
  • Employing native genetic regulatory elements for expression control.

Main Results:

  • Successfully controlled protein levels using synthetic genes with adapted codons in C. elegans.
  • Established a correlation between the amount of G-protein regulator GPR-1/2 and spindle force in C. elegans embryos.
  • Demonstrated the versatility of the codon adaptation method for various C. elegans genes.

Conclusions:

  • Codon-adapted synthetic genes provide a powerful tool for precise control of protein levels in C. elegans.
  • The developed method facilitates functional studies of genes involved in developmental processes.
  • The C. elegans codon adapter web tool enables accessible design of custom synthetic genes.