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Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...

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Analyzing Large Protein Complexes by Structural Mass Spectrometry
15:35

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

Miniaturization of Mass Spectrometry Analysis Systems.

Wei Xu1, Nicholas E Manicke, Graham R Cooks

  • 1Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA.

JALA (Charlottesville, Va.)
|February 1, 2011
PubMed
Summary
This summary is machine-generated.

Researchers developed miniaturized mass spectrometry systems for portable chemical analysis. These systems utilize ambient ionization and compact mass analyzers for rapid, on-site sample testing without extensive preparation.

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Last Updated: Jun 4, 2026

Analyzing Large Protein Complexes by Structural Mass Spectrometry
15:35

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Published on: June 19, 2010

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
07:55

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Published on: June 21, 2019

Area of Science:

  • Analytical Chemistry
  • Instrument Development
  • Spectrometry

Background:

  • Miniaturization of analytical instrumentation is crucial for portable and on-site analysis.
  • Traditional mass spectrometry systems are often large, complex, and require significant sample preparation.
  • Developing compact and user-friendly mass spectrometers is a key challenge in analytical science.

Purpose of the Study:

  • To introduce key technologies for miniaturizing mass spectrometry (MS) systems.
  • To demonstrate the capabilities of novel atmospheric pressure interfaces and ambient ionization methods for MS.
  • To showcase the performance of handheld MS systems for direct chemical analysis.

Main Methods:

  • Development of miniaturized mass analyzers with simple geometries.
  • Integration of a novel atmospheric pressure interface for ion trap mass spectrometers.
  • Coupling of ambient ionization techniques (desorption electrospray ionization, low temperature plasma probe, paper spray) to miniature MS.
  • Evaluation of two handheld MS systems: Mini 10 and Mini 11.

Main Results:

  • Successful reduction in the size of ion trap mass spectrometers.
  • Demonstration of direct MS analysis of complex samples using ambient ionization.
  • No requirement for sample preparation or chemical separation with developed methods.
  • Characterization of the performance and capabilities of handheld MS systems for chemical analysis.

Conclusions:

  • Miniaturized mass spectrometry systems are feasible for portable and on-site chemical analysis.
  • Ambient ionization techniques coupled with compact MS enable rapid analysis of complex samples.
  • The developed technologies and handheld systems offer a promising platform for diverse analytical applications.