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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

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Updated: Jun 4, 2026

Massively Parallel Reporter Assays in Cultured Mammalian Cells
11:03

Massively Parallel Reporter Assays in Cultured Mammalian Cells

Published on: August 17, 2014

CEST MRI reporter genes.

Guanshu Liu1, Jeff W M Bulte, Assaf A Gilad

  • 1Kennedy Krieger Institute, F.M. Kirby Research Center for Functional Brain Imaging, The Johns Hopkins University School of Medicine, Baltimore, MD, USA. guanshu@mri.jhu.edu

Methods in Molecular Biology (Clifton, N.J.)
|February 1, 2011
PubMed
Summary
This summary is machine-generated.

New reporter genes enable non-invasive magnetic resonance imaging (MRI). This study details protocols for chemical exchange saturation transfer (CEST) reporter gene cloning, expression, and imaging.

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Genomic MRI - a Public Resource for Studying Sequence Patterns within Genomic DNA
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Published on: May 9, 2011

Area of Science:

  • Biomedical Imaging
  • Molecular Biology
  • Biotechnology

Background:

  • Reporter genes are crucial for non-invasive imaging.
  • Magnetic resonance imaging (MRI) offers high spatial resolution and soft-tissue contrast.
  • Developing effective reporter genes for MRI is an active area of research.

Purpose of the Study:

  • To summarize recent advancements in MRI reporter gene technology.
  • To provide detailed protocols for chemical exchange saturation transfer (CEST) reporter gene applications.
  • To facilitate the use of CEST-based reporter genes for non-invasive imaging.

Main Methods:

  • Review of recent literature on MRI reporter gene development.
  • Detailed protocols for cloning CEST reporter genes.
  • Protocols for expression and non-invasive imaging of CEST reporter genes.

Main Results:

  • Recent progress in developing reporter genes for MRI was summarized.
  • Practical "hands-on" protocols for CEST reporter gene technology were established.
  • The study provides a foundation for utilizing CEST reporter genes in various biological applications.

Conclusions:

  • CEST-based reporter genes represent a promising tool for non-invasive MRI.
  • The provided protocols can accelerate research in molecular imaging.
  • Further development in this field holds potential for improved diagnostics and therapeutics.