Related Experiment Video

Updated: Jun 4, 2026

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
12:20

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

Involvement of cyclophilin B in the replication of Japanese encephalitis virus

Hiroto Kambara1, Hideki Tani, Yoshio Mori

  • 1Department of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita, Osaka, Japan

Virology
|February 2, 2011
PubMed

Insights

Cyclosporin A inhibits Japanese encephalitis virus (JEV) by targeting CypB. This protein

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Japanese encephalitis virus (JEV), a Flaviviridae family member, causes significant neurological disease.
  • Understanding JEV replication mechanisms is crucial for developing antiviral strategies.

Purpose of the Study:

  • To investigate the effect of cyclosporin A (CsA) on JEV propagation.
  • To elucidate the role of cyclophilin B (CypB) in JEV replication.

Main Methods:

  • Cell-based assays using various mammalian cell lines.
  • Cyclosporin A treatment and CypB knockdown experiments.
  • Analysis of JEV propagation, viral protein interactions, and cyclophilin B peptidylprolyl cis-trans isomerase (PPIase) activity.

Main Results:

  • Cyclosporin A demonstrated potent anti-JEV activity by inhibiting CypB.
  • JEV propagation was significantly reduced in CypB-knockdown cells.
  • CypB's PPIase activity, not its mere presence, is essential for JEV replication, as indicated by rescue experiments with wild-type vs. PPIase-deficient CypB.
  • CypB interacts with JEV NS4A protein, suggesting a role in viral replication, not entry.

Conclusions:

  • CypB is a critical host factor for JEV replication.
  • The peptidylprolyl cis-trans isomerase (PPIase) activity of CypB is essential for JEV propagation.
  • CypB interacts with JEV NS4A, highlighting a potential therapeutic target for JEV infection.

More Related Videos

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry09:38

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

Published on: June 26, 2019

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle09:35

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Published on: February 1, 2017

Related Concept Videos

Encephalitis ll: Pathophysiology01:26

Encephalitis ll: Pathophysiology

Encephalitis is inflammation of the brain parenchyma caused by direct viral invasion or immune-mediated mechanisms triggered by infections or tumors. Both processes lead to neuronal injury, disrupted neurotransmission, and diverse neurological symptoms, often with overlapping clinical and pathological features.Autoimmune EncephalitisIn autoimmune encephalitis, antibodies target neuronal antigens on cell surfaces, synapses, or within neurons. A key example is anti-NMDAR encephalitis, which can...
Arboviral Encephalitis01:25

Arboviral Encephalitis

Arboviral encephalitis refers to brain inflammation caused by arthropod-borne viruses, particularly those transmitted through mosquito vectors. Among these, West Nile virus (WNV), a member of the Flaviviridae family, is a significant public health concern. WNV is an enveloped, positive-sense, single-stranded RNA virus. Human infection typically begins when an infected mosquito introduces the virus into the dermis during feeding. The primary transmission cycle involves birds as amplifying hosts...
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Viral Replication: Lysogenic Cycle01:16

Viral Replication: Lysogenic Cycle

The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects its...
Viral Replication: Lytic Cycle01:20

Viral Replication: Lytic Cycle

Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...