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Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
Published on: November 15, 2013
A metabolomics strategy for detecting protein-metabolite interactions to identify natural nuclear receptor ligands
Yun-Gon Kim1, Angela C Lou, Alan Saghatelian
1Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford St., Cambridge, MA, USA.
Abstract:
Nuclear receptors are ligand-regulated transcription factors that control a range of (patho)physiological processes. Lipids are the most common type of nuclear receptor ligands. Here, we utilize a metabolomics approach that detects protein-metabolite interactions to identify endogenous peroxisome proliferator-activated receptor (PPAR) ligands from tissue and cell extracts.
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