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Related Experiment Video

Updated: Jun 4, 2026

Optical Frequency Domain Imaging of Ex vivo Pulmonary Resection Specimens: Obtaining One to One Image to Histopathology Correlation

Optical Frequency Domain Imaging of Ex vivo Pulmonary Resection Specimens: Obtaining One to One Image to Histopathology Correlation

Published on: January 22, 2013

Field histology: Point-of-care microscopic technique.

Kaumakaokalani Calhoun¹, Alexander Lin, Peter Bryant-Greenwood

¹Department of Pathology, The Queen’s Medical Center, University of Hawaii School of Medicine, Honolulu, USA.

Archives of Pathology & Laboratory Medicine

|February 3, 2011

Summary

This summary is machine-generated.

A new rapid histology technique provides point-of-care diagnostic slides in 1.5 hours. This method is ideal for remote areas and medical missions lacking standard pathology services.

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Optical Frequency Domain Imaging of Ex vivo Pulmonary Resection Specimens: Obtaining One to One Image to Histopathology Correlation

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Published on: August 25, 2020

Area of Science:

Medical diagnostics
Pathology
Point-of-care testing

Background:

Standard histology services are limited in remote areas, causing diagnostic delays.
Rapid tissue processing and portable microtomy can enable point-of-care diagnosis in low-resource settings.

Purpose of the Study:

To introduce an alternative to conventional histology for point-of-care histopathology.

Main Methods:

21 diverse tissue samples underwent rapid manual processing, portable microtome sectioning, and hematoxylin-eosin staining.
Histologic preparations were assessed for diagnostic quality based on section uniformity, architectural integrity, cytologic detail, staining, and overall adequacy.

Main Results:

Diagnostic quality slides were achieved for all 21 samples, including skin, uterus, colon, and breast lesions.
The average preparation time, including staining, was 1.5 hours.

Conclusions:

A point-of-care field histology technique was validated for low-resource settings like medical missions.
Future steps include field testing with special stains for fungi and acid-fast bacilli.