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[Comparative study of 2 rapid techniques (DNA hybridization and chromatography) for the identification of
J M Artero1, E A Herrera, P L Valero
1Servicio de Microbiología, Hospital General de Murcia.
Enfermedades Infecciosas Y Microbiologia Clinica
|October 1, 1990
Abstract:
We have compared the capacity of two combined chromatographic methods (gas chromatography and thin-layer chromatography) and a nucleic acid hybridization probe (Gen-Probe) to identify members of the Mycobacterium tuberculosis complex. The results of a single test show 6% of false-negatives with Gen-Probe and 8.5% doubtfuls with chromatography. After repeated, the specificity of both methods was improved to 100%. The advantages and disadvantages of the two methods in relation to its capacity to provide a rapid identification of Mycobacterium tuberculosis are discussed.