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Related Concept Videos

Nucleosome Remodeling02:54

Nucleosome Remodeling

Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
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Chromatin Modification in iPS Cells01:32

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Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
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Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
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NF-kB-dependent Signaling Pathway

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NF-κB-dependent Signaling Mechanism
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Chromatin Position Affects Gene Expression

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Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
08:44

Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes

Published on: October 23, 2014

Chromatin remodeling complex NURF regulates thymocyte maturation.

Joseph W Landry1, Subhadra Banerjee, Barbara Taylor

  • 1Laboratory of Biochemistry and Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. jwlandry2@vcu.edu

Genes & Development
|February 4, 2011
PubMed
Summary

Thymocyte maturation relies on the nucleosome remodeling factor (NURF). Depleting its subunit, Bptf (bromodomain PHD finger transcription factor), halts T cell development by altering gene expression and chromatin structure.

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Published on: October 23, 2014

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Examination of Thymic Positive and Negative Selection by Flow Cytometry
14:29

Examination of Thymic Positive and Negative Selection by Flow Cytometry

Published on: October 8, 2012

Area of Science:

  • Immunology
  • Molecular Biology
  • Epigenetics

Background:

  • T cell maturation requires cytokine and T-cell receptor signaling to target genes in chromatin.
  • The influence of chromatin architecture on T cell maturation is not well understood.

Purpose of the Study:

  • To investigate the role of chromatin architecture in T cell development.
  • To elucidate the function of the nucleosome remodeling factor (NURF) in thymocyte maturation.

Main Methods:

  • Utilized conditional mouse mutants to deplete Bptf (bromodomain PHD finger transcription factor), a key NURF subunit.
  • Analyzed thymocyte development, gene expression, and chromatin structure (DNase I hypersensitivity).
  • Investigated physical interactions between NURF and transcription factors.

Main Results:

  • Depletion of Bptf caused developmental arrest beyond the CD4(+) CD8(int) stage without affecting proliferation or apoptosis.
  • Aberrant expression of key thymocyte development genes was observed in Bptf mutants.
  • Defects in chromatin structure and Srf (serum response factor) binding at Bptf-dependent genes were identified.

Conclusions:

  • Thymocyte maturation post-positive selection is dependent on NURF, specifically Bptf.
  • Bptf facilitates Srf recruitment to regulatory sites, influencing transcription factor binding and gene regulation.
  • Establishes a link between NURF, transcription factor occupancy, and gene regulation in T cell development.