Related Experiment Video
Updated: Jun 4, 2026

Cultivation Methods of Spirochetes from Borrelia burgdorferi Sensu Lato Complex and Relapsing Fever Borrelia
Published on: November 25, 2022
Combining culture techniques for Bartonella: the best of both worlds
Tarah Lynch1, Jennifer Iverson, Michael Kosoy
1Centers for Disease Control and Prevention, Division of Vector Borne Diseases, 3150 Rampart Road, Fort Collins, CO 80521, USA.
Abstract:
In this study we compared some common Bartonella culturing methodologies using four diverse species causing human illnesses. Based on a review of the literature, we focused on three major inconsistencies between protocols: base medium, cell coculture, and temperature. Our data showed that Bartonella tamiae demonstrated temperature-dependent growth limitations between common culturing conditions only 2°C apart. Additionally, growth of B. quintana was significantly enhanced by the presence of mammalian cell coculture under mammalian cell culture conditions; however, when the medium was modified to incorporate insect cell culture-based medium, coculturing with mammalian cells was no longer needed. In this study, we were able to overcome these temperature- and cell-dependent limitations and accommodate all of the strains tested by combining mammalian cell culture-based medium with insect cell culture-based medium.
Related Concept Videos
Combined Effects of Drugs: Synergism
Such synergistic combinations...
Methods of Classification and Identification
Special Staining Techniques
Key Techniques in Microbiology
Techniques for Isolation of Pure Cultures
Sputum Studies II: Culture and Sensitivity
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...

