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Poly(ethylene oxide), a new slowing agent for protozoa.

D M Spoon, I I Feise CO, R S Youn

    The Journal of Protozoology
    |August 1, 1977
    PubMed
    Summary

    Polyox WSR 301, a high molecular weight poly(ethylene oxide), effectively slows protozoa motility. Unlike other agents, Polyox offers reduced toxicity and unique elastic recoil properties for enhanced biological research applications.

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    Area of Science:

    • Microbiology
    • Biophysics

    Background:

    • Protozoa motility is crucial for their ecological roles.
    • Existing slowing agents like methyl cellulose and Protoslo have limitations.

    Purpose of the Study:

    • To evaluate Polyox WSR 301 as a novel slowing agent for protozoa.
    • To compare its efficacy and toxicity against existing agents.

    Main Methods:

    • Tested Polyox WSR 301 solutions of varying concentrations (0.25%-1% w/v).
    • Measured swimming velocities of Euglena gracilis, Didinium nasutum, Paramecium aurelia, Blepharisma undulans, and Prorodon platyodon.
    • Assessed toxicity using Paramecium aurelia over 96 hours.

    Main Results:

    • Increased Polyox WSR 301 viscosity significantly decreased protozoa swimming velocity.
    • Polyox solutions were more effective at reducing velocity than methyl cellulose and Protoslo.
    • Polyox exhibited lower toxicity, with Paramecium surviving and dividing in lower concentrations.

    Conclusions:

    • Polyox WSR 301 is a superior slowing agent for protozoa due to its effectiveness and low toxicity.
    • Its unique elastic recoil property offers distinct advantages over viscous drag alone.

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