Related Experiment Video
Updated: Jun 4, 2026

Label-Free Surface-Enhanced Raman Scattering Bioanalysis Based on Au@Carbon Dot Nanoprobes
Published on: June 9, 2023
Differential separation of protein mixtures using convective assembly and label-free detection with surface enhanced
Sercan Keskin1, Mehmet Kahraman, Mustafa Culha
1Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Ataşehir, Istanbul 34755, Turkey.
Abstract:
Differential separation and label-free detection of proteins using SERS from their mixtures after a convective assembly process is reported. Binary and ternary mixtures of proteins are mixed with AgNPs and assembled into ordered structures. The spectra acquired from the assembled area indicate that the proteins are differentially distributed.
More Related Videos
08:04A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
Published on: March 13, 2014
10:00Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)
Published on: June 20, 2019
Related Concept Videos
SDS-PAGE
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...