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Related Concept Videos

Hypoxia01:23

Hypoxia

Hypoxia is a medical condition characterized by an inadequate oxygen supply to body tissues. It typically manifests as a bluish discoloration of the skin and mucosae, especially in fair-skinned individuals, when hemoglobin (Hb) saturation drops below 75%.
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...
Glucose Homeostasis: Pancreatic Islets and Insulin Secretion01:27

Glucose Homeostasis: Pancreatic Islets and Insulin Secretion

The pancreatic islets comprising only 1%-2% of the volume are highly vascularized and innervated mini-organs. They contain five endocrine cell types, including β cells that secrete insulin, which is synthesized as a single polypeptide chain, preproinsulin, processed to proinsulin, and finally to insulin and C-peptide. This process is complex and regulated, involving the Golgi complex, the endoplasmic reticulum, and the secretory granules of the β cell.
Insulin and C-peptide are co-secreted in...

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Tracking Hypoxic Signaling within Encapsulated Cell Aggregates
09:14

Tracking Hypoxic Signaling within Encapsulated Cell Aggregates

Published on: December 16, 2011

Correlating hypoxia with insulin secretion using a fluorescent hypoxia detection system.

Matthew L Skiles1, Romone Fancy, Pritesh Topiwala

  • 1Biomedical Engineering Program, University of South Carolina College of Engineering and Computing, Columbia, South Carolina, USA. skiles@email.sc.edu

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
|February 4, 2011
PubMed
Summary

Hypoxia, or oxygen deficiency, threatens encapsulated pancreatic cells. A new fluorescent marker system detects this stress early, predicting insulin release and improving tissue survival research.

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Tracking Hypoxic Signaling within Encapsulated Cell Aggregates
09:14

Tracking Hypoxic Signaling within Encapsulated Cell Aggregates

Published on: December 16, 2011

Quantitative and Temporal Control of Oxygen Microenvironment at the Single Islet Level
11:49

Quantitative and Temporal Control of Oxygen Microenvironment at the Single Islet Level

Published on: November 17, 2013

Induction and Testing of Hypoxia in Cell Culture
07:01

Induction and Testing of Hypoxia in Cell Culture

Published on: August 12, 2011

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Tissue Engineering

Background:

  • Oxygen insufficiency is a major challenge for encapsulated tissue survival, especially pancreatic beta-cells.
  • Early detection of hypoxic stress is crucial for maintaining cell function and viability in engineered tissues.

Purpose of the Study:

  • To investigate a novel fluorescent hypoxia detection system for early recognition of hypoxic stress in encapsulated pancreatic tissue.
  • To assess the impact of varying cell densities and oxygen levels on encapsulated MIN6 cell function and marker expression.

Main Methods:

  • Engineered murine insulinoma (MIN6) cells to express red fluorescent protein regulated by hypoxia-inducible-factor-1.
  • Encapsulated MIN6 cell aggregates in poly(ethylene glycol) hydrogels at different densities.
  • Incubated encapsulated cells in low (1%) and normal (20%) oxygen environments.
  • Monitored cell function via glucose-stimulated insulin secretion and fluorescent imaging of the hypoxia marker.

Main Results:

  • Oxygen insufficiency significantly impaired insulin release from MIN6 cells.
  • Larger cell aggregates were more vulnerable to oxygen limitations.
  • The developed fluorescent marker accurately indicated hypoxia and predicted subsequent insulin release.

Conclusions:

  • The fluorescent hypoxia detection system is a valuable tool for early recognition of hypoxic stress in encapsulated tissues.
  • This method can predict functional decline and may improve strategies for tissue survival in various biological research fields.