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Updated: Jun 4, 2026

Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension
Published on: September 11, 2020
Asymmetrical flow field-flow fractionation technique for separation and characterization of biopolymers and
G Yohannes1, M Jussila, K Hartonen
1Laboratory of Analytical Chemistry, Department of Chemistry, P.O. Box 55, FIN-00014, University of Helsinki, Finland.
Abstract:
Field-flow fractionation (FFF) is one of the most versatile separation techniques in the field of analytical separation sciences, capable of separating macromolecules in the range 10³-10¹⁵ g mol⁻¹ and/or particles with 1 nm-100 μm in diameter. The most universal and most frequently used FFF technique, flow FFF, includes three types of techniques, namely symmetrical flow FFF, hollow fiber flow FFF, and asymmetrical flow FFF which is most established variant among them. This review provides a brief look at the theoretical background of analyte retention and separation efficiency in FFF, followed by a comprehensive overview of the current status of asymmetrical flow FFF with selected applications in the field of biopolymers and bioparticles.
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