Related Experiment Video
Updated: Jun 4, 2026

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
Published on: July 17, 2021
Insertion-/deletion polymorphisms close to the repeat region of STR loci can cause discordant genotypes with
Burkhard Rolf1, Nicole Bulander, Peter Wiegand
1Eurofins Medigenomix GmbH, Anzinger Str. 7a, D-85560 Ebersberg, Germany. BurkhardRolf@eurofins.com
Abstract:
It is well known that mutations in the primer binding site of STR loci can cause "null alleles". If a sample is typed with different sets of PCR primers (for example if different kits or kits of different manufacturers are used) discordant results might be observed, thus making the use of different STR kits for deviant genotypes necessary. Here we present another reason for using different kits: Insertion-/deletion polymorphism between the repeat region of the STR locus and the primer binding site can also cause genotypes that vary between different kits/primer sets.
Related Concept Videos
Single Nucleotide Polymorphisms-SNPs
Comparing Copy Number Variations and SNPs
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
Gene Conversion
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Genome Copying Errors
