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RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

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Types of RNA

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

Types of RNA

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
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DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

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Published on: December 29, 2021

Combinatorially inducible RNA interference triggered by chemically modified oligonucleotides.

Deepak Kumar¹, Sang Hoon Kim, Yohei Yokobayashi

¹Department of Biomedical Engineering, University of California, Davis, Davis, California 95616, USA.

Journal of the American Chemical Society

|February 8, 2011

Summary

This study introduces a novel chemically inducible RNA interference (RNAi) platform for precise gene silencing. This system enables combinatorial control of multiple genes, accelerating research in gene function and drug discovery.

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Area of Science:

Molecular Biology
Gene Regulation
Biotechnology

Background:

Chemically inducible RNA interference (RNAi) offers precise control over gene expression for various applications.
Existing RNAi methods may lack the combinatorial control needed for complex gene interaction studies.

Purpose of the Study:

To develop a new inducible RNAi platform for combinatorial gene silencing.
To enable temporal and spatial control of gene expression using chemical inducers.

Main Methods:

Designed a modular RNA architecture with an oligonucleotide sensor stem-loop and an RNAi effector domain.
Utilized orthogonal chemically modified oligonucleotides as inducers to trigger RNA structural changes.
Leveraged RNAi machinery for gene silencing upon structural shift.

Main Results:

Successfully demonstrated a novel inducible RNAi platform.
Showcased the ability to trigger gene silencing through chemically induced structural changes in RNA.
Established a system for combinatorial regulation of two genes.

Conclusions:

The developed platform provides a powerful tool for studying complex gene interactions.
This approach accelerates the screening of potential drug targets.
Offers potential applications in gene therapy and functional genomics research.