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Updated: Jun 4, 2026
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Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression
Published on: June 15, 2019
Cecal ligation and puncture: the gold standard model for polymicrobial sepsis?
Lien Dejager1, Iris Pinheiro, Eline Dejonckheere
1Department for Molecular Biomedical Research, Flanders Institute for Biotechnology (VIB), B9052 Ghent, Belgium.
Trends in Microbiology
|February 8, 2011
Summary
Sepsis research using cecal ligation and puncture (CLP) models offers insights into immune responses but faces translation challenges. Future strategies should address sepsis-induced immunosuppression and patient heterogeneity for better clinical outcomes.
Area of Science:
- Immunology
- Pathophysiology
- Medical Research
Background:
- Sepsis involves systemic inflammation and subsequent immunosuppression.
- Animal models are crucial for studying sepsis pathophysiology.
- Cecal ligation and puncture (CLP) is a widely used model for polymicrobial sepsis.
Purpose of the Study:
- To summarize the role of immune components in CLP-induced sepsis.
- To highlight the limitations of experimental sepsis models in mimicking human sepsis.
- To propose strategies for improving the clinical translation of sepsis research.
Main Methods:
- Review of immune components in CLP-induced sepsis.
- Analysis of discrepancies between CLP model findings and clinical sepsis outcomes.
- Identification of key factors for enhancing experimental model relevance.
Main Results:
- The CLP model effectively mimics aspects of human sepsis progression and immune response.
- Therapeutic strategies successful in CLP models often fail in clinical trials.
- Significant gaps exist between experimental sepsis models and human sepsis complexity.
Conclusions:
- Experimental sepsis models, particularly CLP, are valuable but imperfect.
- Addressing the immunosuppressive phase and patient heterogeneity is key to bridging the research-clinic gap.
- Improved models are needed to develop effective sepsis therapies.
