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Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases
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Detection of circulating endothelial cells via a microfluidic disk.

Ken-Chao Chen1, Tai-Ping Lee, Yu-Cheng Pan

  • 1Institute of Applied Mechanics, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan.

Clinical Chemistry
|February 8, 2011
PubMed
Summary

Detecting rare circulating endothelial cells (CECs) is challenging. This study introduces an economical microfluidic disk for CEC detection, showing higher counts in systemic lupus erythematosus patients, indicating its clinical viability.

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Clinical Diagnostics

Background:

  • Circulating endothelial cells (CECs) are rare blood markers linked to diseases.
  • Detecting CECs amidst peripheral blood mononuclear cells (PBMCs) presents significant challenges due to low ratios.
  • A novel microfluidic disk system is developed for efficient CEC isolation and detection.

Purpose of the Study:

  • To develop an economical microfluidic disk for detecting circulating endothelial cells (CECs).
  • To validate the system's efficacy using a model cell system and clinical samples.
  • To assess the diagnostic potential of CEC counts in systemic lupus erythematosus (SLE).

Main Methods:

  • Utilized immunomagnetic beads and a custom magnet on a microfluidic disk for cell enrichment.
  • Employed specific antibodies (anti-CD146-phycoerythrin for CECs, anti-CD45-FITC for PBMCs) for cell labeling.
  • Validated detection efficiency with model cells (HUVECs in PBMCs) and compared results with flow cytometry.

Main Results:

  • Achieved 95% detection of spiked human umbilical vein endothelial cells (HUVECs) in PBMCs.
  • Demonstrated high yield even at low cell ratios (60 HUVECs in <10^4 PBMCs).
  • Found significantly higher CEC counts in SLE patients (61.1) compared to healthy donors (31.2).

Conclusions:

  • CEC count serves as a suitable biomarker for systemic lupus erythematosus symptoms.
  • The developed microfluidic disk system offers a viable platform for CEC detection.
  • This technology has potential for broader clinical diagnostic applications.