Related Experiment Video
Updated: Jun 4, 2026
07:30
Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion
Published on: May 10, 2018
Competitive solid-phase immunoassay of gastrin in serum using time-resolved fluorometry.
Anders H Johnsen1, Fahed N Assaad, Jens F Rehfeld
1Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Denmark. anders.johnsen@rh.regionh.dk
Scandinavian Journal of Clinical and Laboratory Investigation
|February 9, 2011
Summary
A new time-resolved fluorescence assay offers a reliable alternative for measuring gastrin in serum, replacing conventional radioimmunoassay (RIA) technology with comparable accuracy and performance.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Immunoassay Technology
Background:
- Conventional radioimmunoassay (RIA) for gastrin measurement faces limitations.
- Development of alternative diagnostic technologies is crucial for clinical laboratories.
Purpose of the Study:
- To develop and validate a competitive solid-phase assay for serum gastrin measurement.
- To establish time-resolved fluorescence as a viable alternative to RIA.
Main Methods:
- Utilized a competitive binding assay with Europium (Eu)-labeled gastrin and gastrin antibodies.
- Employed solid-phase technology with immobilized antibodies on microtitration strips.
- Measured bound Eu(3+) label via time-resolved fluorometry after chelation.
Main Results:
- Achieved a lower limit of detection below 10 pmol/L with a 50 μl sample volume.
- Demonstrated excellent linearity upon sample dilution and 103% recovery in spiking studies.
- Showed full correlation with routine radioimmunoassay (RIA) in the 10-400 pmol/L range (r²=0.98).
Conclusions:
- A convenient and accurate competitive assay for serum gastrin measurement was developed.
- Time-resolved fluorometric detection offers a realistic alternative to established RIA technology.
- The new assay allows for seamless transition from RIA without altering reference intervals.
