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Isolation of high-quality RNA from various tissues of Jatropha curcas for downstream applications

G Raja Krishna Kumar1, Nalini Eswaran, T Sudhakar Johnson

  • 1Plant Metabolic Engineering Group, Reliance Life Sciences, Rabale, Navi Mumbai 400 701, India.

Analytical Biochemistry
|February 9, 2011
PubMed

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RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...