Related Experiment Video

Updated: Jun 4, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
06:34

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Published on: July 11, 2016

Identification of Staphylococcus intermedius Group by MALDI-TOF MS

Paola Decristophoris1, Amy Fasola, Cinzia Benagli

  • 1Cantonal Institute of Microbiology, 6500 Bellinzona, Switzerland. paola.decristophoris@ti.ch

Systematic and Applied Microbiology
|February 9, 2011
PubMed

Insights

Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) accurately identifies Staphylococcus intermedius Group bacteria. This proteomic method offers a reliable alternative to molecular techniques for rapid bacterial identification.

Area of Science:

  • Microbiology
  • Bacteriology
  • Proteomics

Background:

  • The Staphylococcus intermedius Group (SIG) comprises coagulase-positive bacteria commonly found in animals.
  • Current identification of SIG relies on molecular methods, which can be time-consuming.
  • There is a need for rapid and accurate diagnostic tools for SIG identification.

Purpose of the Study:

  • To evaluate the suitability of MALDI-TOF MS for identifying SIG bacteria.
  • To compare the performance of MALDI-TOF MS against partial hsp60 gene sequencing.
  • To assess the sensitivity, specificity, and efficiency of MALDI-TOF MS for SIG identification.

Main Methods:

  • Analysis of 69 SIG strains (S. intermedius, S. pseudintermedius, S. delphini) using MALDI-TOF MS.
  • Identification confirmation via partial hsp60 gene sequencing.
  • Calculation of sensitivity, specificity, efficiency, and Cohen's kappa coefficient for MALDI-TOF MS.

Main Results:

  • MALDI-TOF MS demonstrated high sensitivity for S. intermedius (0.95) and high specificity for S. intermedius and S. delphini (1.0).
  • Overall efficiency ranged from 0.88 (S. pseudintermedius, S. delphini) to 0.99 (S. intermedius).
  • Almost perfect agreement (kappa=0.96) was observed between MALDI-TOF MS and hsp60 sequencing for S. intermedius.

Conclusions:

  • MALDI-TOF MS is a valuable and reliable tool for rapid and accurate identification of SIG bacteria.
  • The proteomic approach offers a significant advancement over traditional molecular methods.
  • MALDI-TOF MS provides efficient and precise identification crucial for veterinary diagnostics.

More Related Videos

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)09:43

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)

Published on: October 30, 2016

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry13:29

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry

Published on: May 28, 2014

Related Concept Videos

Rapid Identification of Pathogens01:25

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...