Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A rat model to study the role of STn antigen in colon cancer.

Glycoconjugate journal·2003

Same author

MUC1-specific CTLs are non-functional within a pancreatic tumor microenvironment.

Glycoconjugate journal·2003

Same author

Probing the conformational and dynamical effects of O-glycosylation within the immunodominant region of a MUC1 peptide tumor antigen.

The journal of peptide research : official journal of the American Peptide Society·2003

Same author

Glycosylations versus conformational preferences of cancer associated mucin core.

Glycoconjugate journal·2001

Same author

Association of MUC-1 and SPGL-1 with low-density microdomain in T-lymphocytes: a preliminary note.

Biochemical and biophysical research communications·2001

Same author

Anti-MUC-1 immunoliposomal doxorubicin in the treatment of murine models of metastatic breast cancer.

Biochimica et biophysica acta·2001

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues

In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues

Published on: September 22, 2017

Histocompatibility typing by cellular radioimmunoassay.

B M Longenecker¹, B Singh, M Gallatin

¹Department of Immunology, The University of Alberta, 845E Medical Sciences Building, T6G 2H7, Edmonton, Alberta, Canada.

|February 9, 2011

Summary

A new quantitative cellular radioimmunoassay (CRIA) offers highly sensitive histocompatibility typing. This method accurately detects minor cell populations and erythrocyte chimerism in chickens.

More Related Videos

Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector

Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8⁺ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector

Published on: November 28, 2018

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

Published on: August 12, 2017

Related Experiment Videos

Last Updated: Jun 4, 2026

In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues

In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues

Published on: September 22, 2017

Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector

Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8⁺ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector

Published on: November 28, 2018

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

Published on: August 12, 2017

Area of Science:

Immunology
Cell Biology
Biochemistry

Background:

Histocompatibility typing is crucial for transplantation and genetic studies.
Conventional methods like hemagglutination assays lack sensitivity and objectivity.
There is a need for more sensitive and precise assays to detect minor cell populations and chimerism.

Purpose of the Study:

To describe a novel quantitative cellular radioimmunoassay (CRIA) for histocompatibility typing.
To evaluate the sensitivity and consistency of the CRIA assay.
To demonstrate the utility of CRIA in detecting erythrocyte chimerism and tumor-specific antigens.

Main Methods:

Chicken red blood cells (RBC) were incubated with specific anti-MHC (B) alloantisera in microtiter plates.
Bound alloantibody was measured indirectly using (125)I-labeled rabbit anti-chicken IgG.
CRIA was applied to artificial cell mixtures, induced erythrocyte chimerism in chickens, and a T-cell lymphoma model.

Main Results:

The CRIA assay demonstrated high sensitivity, detecting as little as 1% of relevant cells in mixtures.
Erythrocyte chimerism in chickens was precisely quantitated, with percentages ranging from 13-40% in chimeric animals.
The assay successfully detected tumor-specific antigens and revealed unexpected binding patterns of anti-B(15) alloantibody.

Conclusions:

CRIA is a highly sensitive, objective, and consistent method for histocompatibility typing.
The assay is effective for quantitating erythrocyte chimerism and detecting minor cell subpopulations.
CRIA has potential applications in tumor immunology and understanding alloantigen expression.