Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

Computed Tomography

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...

Imaging Studies III: Computed Tomography

Imaging Studies III: Computed Tomography

DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...

Two-Dimensional Microscopy in Microbiology

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

Total Internal Reflection Fluorescence Microscopy

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Feasibility of using geometry-based synthetic dose distributions for Knowledge-based planning model training.

Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB)·2026

Same author

A multimodal platform for real-time neurochemical and electrophysiologic monitoring for intraoperative neurosurgical applications.

Biosensors & bioelectronics·2025

Same author

Improved circuitry and post-processing for interleaved fast-scan cyclic voltammetry and electrophysiology measurements.

Frontiers in signal processing·2024

Same author

Measurement of enhanced vasa vasorum density in a porcine carotid model using photon counting detector CT.

Journal of medical imaging (Bellingham, Wash.)·2023

Same author

Overcoming calcium blooming and improving the quantification accuracy of percent area luminal stenosis by material decomposition of multi-energy computed tomography datasets.

Journal of medical imaging (Bellingham, Wash.)·2020

Same author

Erratum to "Impact of Effective Detector Pixel and CT Voxel Size on Accurate Estimation of Blood Volume in Opacified Microvasculature" [Acad Radiol 2019; 26:1410-1416].

Academic radiology·2020

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Published on: May 27, 2008

Direct three-dimensional coherently scattered x-ray microtomography.

Congwu Cui¹, Steven M Jorgensen, Diane R Eaker

¹Department of Medical Physics, CancerCare Manitoba, 675 McDermot Avenue, Winnipeg, Manitoba R3E 0V9, Canada.

Medical Physics

|February 10, 2011

Summary

This study introduces a novel coherently scattered X-ray tomography method for identifying components in low atomic weight materials. The technique allows for direct 3D imaging of small biological samples, potentially achieving submillimeter resolution.

More Related Videos

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT

Published on: June 21, 2011

Related Experiment Videos

Last Updated: Jun 4, 2026

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Published on: May 27, 2008

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT

Published on: June 21, 2011

Area of Science:

Materials Science
Medical Imaging
Physics

Background:

Coherently scattered X-rays offer potential for material discrimination.
Previous methods required complex tomographic reconstruction.
Low atomic weight materials present challenges in conventional imaging.

Purpose of the Study:

To demonstrate a new method for coherently scattered X-ray tomography.
To enable component identification in low atomic weight material mixtures.
To develop a simplified tomographic approach using a thin X-ray sheet.

Main Methods:

Utilized a collimated X-ray fan-beam and polycapillary collimator.
Employed a phantom with low attenuation-based contrast biocompatible materials.
Investigated contrast changes with momentum transfer using various X-ray sources (Cu/Ni, Mo/Zr, Ag/Pd) and scatter angles.

Main Results:

Material contrast varied with X-ray source energy and measurement angle.
Coherent scatter profiles aligned with published data.
The simplified experimental setup proved feasible.

Conclusions:

The method can generate 3D coherent scatter images of small objects.
Suitable for low atomic weight biological and synthetic materials.
Submillimeter spatial resolution is achievable with optimized equipment.